HUYNH LAB
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RESEARCH
The Huynh Lab is located in Duncan Hall at San José State University. We study how nutrients are metabolized and how this contributes to overall health. The way nutrients are metabolized and stored can have profound effects on most physiological processes. The Huynh Lab uses a multidisciplinary approach to study how carbohydrate, lipid, and amino acid metabolic pathways are regulated, how they interact, and how dysregulation of these pathways can lead to diseases such as diabetes and obesity. We are particularly interested in how metabolic pathways are regulated by hormones and post-translational modifications.
PUBLICATIONS
For an updated list of publications, please visit:
https://scholar.google.com/citations?user=yoy42lAAAAAJ&hl=en
Huynh, F. K., Peterson, B. S., Anderson, K. A., Lin, Z., Coakley, A. J., Llaguno, F., Nguyen, T. N., Campbell, J. E., Stephens, S. B., Newgard, C. B., & Hirschey, M. D. (2020). β-Cell-specific ablation of sirtuin 4 does not affect nutrient-stimulated insulin secretion in mice. American journal of physiology. Endocrinology and metabolism, 319(4), E805–E813. https://doi.org/10.1152/ajpendo.00170.2020
Naiman, S., Huynh, F. K., Gil, R., Glick, Y., Shahar, Y., Touitou, N., Nahum, L., Avivi, M. Y., Roichman, A., Kanfi, Y., Gertler, A. A., Doniger, T., Ilkayeva, O. R., Abramovich, I., Yaron, O., Lerrer, B., Gottlieb, E., Harris, R. A., Gerber, D., Hirschey, M. D., Cohen, H. Y. (2019). SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα. Cell reports, 29(12), 4127–4143.e8. https://doi.org/10.1016/j.celrep.2019.11.067
Tudurí, E., Glavas, M. M., Asadi, A., Baker, R. K., Ellis, C. E., Soukhatcheva, G., Philit, M., Huynh, F. K., Johnson, J. D., Bruce Verchere, C., & Kieffer, T. J. (2019). AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells. Scientific reports, 9(1), 10829. https://doi.org/10.1038/s41598-019-46735-2
Ali, H. R., Assiri, M. A., Harris, P. S., Michel, C. R., Yun, Y., Marentette, J. O., Huynh, F. K., Orlicky, D. J., Shearn, C. T., Saba, L. M., Reisdorph, R., Reisdorph, N., Hirschey, M. D., & Fritz, K. S. (2019). Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism. Journal of proteome research, 18(4), 1513–1531. https://doi.org/10.1021/acs.jproteome.8b00800
Nicholatos, J. W., Robinette, T. M., Tata, S., Yordy, J. D., Francisco, A. B., Platov, M., Yeh, T. K., Ilkayeva, O. R., Huynh, F. K., Dokukin, M., Volkov, D., Weinstein, M. A., Boyko, A. R., Miller, R. A., Sokolov, I., Hirschey, M. D., & Libert, S. (2019). Cellular energetics and mitochondrial uncoupling in canine aging. GeroScience, 41(2), 229–242. https://doi.org/10.1007/s11357-019-00062-6
Huynh, F. K., Hu, X., Lin, Z., Johnson, J. D., & Hirschey, M. D. (2018). Loss of sirtuin 4 leads to elevated glucose- and leucine-stimulated insulin levels and accelerated age-induced insulin resistance in multiple murine genetic backgrounds. Journal of inherited metabolic disease, 41(1), 59–72. https://doi.org/10.1007/s10545-017-0069-8
Anderson, K. A., Huynh, F. K., Fisher-Wellman, K., Stuart, J. D., Peterson, B. S., Douros, J. D., Wagner, G. R., Thompson, J. W., Madsen, A. S., Green, M. F., Sivley, R. M., Ilkayeva, O. R., Stevens, R. D., Backos, D. S., Capra, J. A., Olsen, C. A., Campbell, J. E., Muoio, D. M., Grimsrud, P. A., & Hirschey, M. D. (2017). SIRT4 Is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion. Cell metabolism, 25(4), 838–855.e15. https://doi.org/10.1016/j.cmet.2017.03.003
Weir, H. J., Yao, P., Huynh, F. K., Escoubas, C. C., Goncalves, R. L., Burkewitz, K., Laboy, R., Hirschey, M. D., & Mair, W. B. (2017). Dietary Restriction and AMPK Increase Lifespan via Mitochondrial Network and Peroxisome Remodeling. Cell metabolism, 26(6), 884–896.e5. https://doi.org/10.1016/j.cmet.2017.09.024
Madsen, A. S., Andersen, C., Daoud, M., Anderson, K. A., Laursen, J. S., Chakladar, S., Huynh, F. K., Colaço, A. R., Backos, D. S., Fristrup, P., Hirschey, M. D., & Olsen, C. A. (2016). Investigating the Sensitivity of NAD+-dependent Sirtuin Deacylation Activities to NADH. The Journal of biological chemistry, 291(13), 7128–7141. https://doi.org/10.1074/jbc.M115.668699
Burkewitz, K., Morantte, I., Weir, H., Yeo, R., Zhang, Y., Huynh, F. K., Ilkayeva, O. R., Hirschey, M. D., Grant, A. R., & Mair, W. B. (2015). Neuronal CRTC-1 governs systemic mitochondrial metabolism and lifespan via a catecholamine signal. Cell, 160(5), 842–855. https://doi.org/10.1016/j.cell.2015.02.004
Huynh, F. K., Muoio, D. M., & Hirschey, M. D. (2015). SIRT3 Directs Carbon Traffic in Muscle to Promote Glucose Control. Diabetes, 64(9), 3058–3060. https://doi.org/10.2337/db15-0709
Anderson, K. A., Green, M. F., Huynh, F. K., Wagner, G. R., & Hirschey, M. D. (2014). SnapShot: Mammalian Sirtuins. Cell, 159(4), 956–956.e1. https://doi.org/10.1016/j.cell.2014.10.045
Huynh, F. K., Green, M. F., Koves, T. R., & Hirschey, M. D. (2014). Measurement of fatty acid oxidation rates in animal tissues and cell lines. Methods in enzymology, 542, 391–405. https://doi.org/10.1016/B978-0-12-416618-9.00020-0
Huynh, F. K., Hershberger, K. A., & Hirschey, M. D. (2013). Targeting sirtuins for the treatment of diabetes. Diabetes management (London, England), 3(3), 245–257. https://doi.org/10.2217/dmt.13.6
Huynh, F. K., Neumann, U. H., Wang, Y., Rodrigues, B., Kieffer, T. J., & Covey, S. D. (2013). A role for hepatic leptin signaling in lipid metabolism via altered very low density lipoprotein composition and liver lipase activity in mice. Hepatology (Baltimore, Md.), 57(2), 543–554. https://doi.org/10.1002/hep.26043
Denroche, H. C., Huynh, F. K., & Kieffer, T. J. (2012). The role of leptin in glucose homeostasis. Journal of diabetes investigation, 3(2), 115–129. https://doi.org/10.1111/j.2040-1124.2012.00203.x
Levi, J., Huynh, F. K., Denroche, H. C., Neumann, U. H., Glavas, M. M., Covey, S. D., & Kieffer, T. J. (2012). Hepatic leptin signalling and subdiaphragmatic vagal efferents are not required for leptin-induced increases of plasma IGF binding protein-2 (IGFBP-2) in ob/ob mice. Diabetologia, 55(3), 752–762. https://doi.org/10.1007/s00125-011-2426-8
Denroche, H. C., Levi, J., Wideman, R. D., Sequeira, R. M., Huynh, F. K., Covey, S. D., & Kieffer, T. J. (2011). Leptin therapy reverses hyperglycemia in mice with streptozotocin-induced diabetes, independent of hepatic leptin signaling. Diabetes, 60(5), 1414–1423. https://doi.org/10.2337/db10-0958
Levi, J., Gray, S. L., Speck, M., Huynh, F. K., Babich, S. L., Gibson, W. T., & Kieffer, T. J. (2011). Acute disruption of leptin signaling in vivo leads to increased insulin levels and insulin resistance. Endocrinology, 152(9), 3385–3395. https://doi.org/10.1210/en.2011-0185
Huynh, F. K., Levi, J., Denroche, H. C., Gray, S. L., Voshol, P. J., Neumann, U. H., Speck, M., Chua, S. C., Covey, S. D., & Kieffer, T. J. (2010). Disruption of hepatic leptin signaling protects mice from age- and diet-related glucose intolerance. Diabetes, 59(12), 3032–3040. https://doi.org/10.2337/db10-0074
Covey, S. D., Wideman, R. D., McDonald, C., Unniappan, S., Huynh, F., Asadi, A., ... & Kieffer, T. J. (2006). The pancreatic β cell is a key site for mediating the effects of leptin on glucose homeostasis. Cell metabolism, 4(4), 291-302. https://doi.org/10.1016/j.cmet.2006.09.005
PEOPLE
Evan Do
Graduate Student
B.S. Biopsychology
University of California, Davis, 2018
Joanne Khau
Graduate Student
B.S. Biological Sciences - Systems Physiology
San Jose State University, 2019
Alison Nguyen
B.A. Biological Science
Adrian Ordonez
B.S. Biological Sciences - Systems Physiology
Minor: Chemistry
LAB ALUMNI
B.S. Biological Sciences - Systems Physiology
Minor: Chemistry
Allyzza Athene Alonzo
Frank K. Huynh
Dr. Frank Huynh obtained a Bachelor of Science in Biochemistry and PhD in Physiology from the University of British Columbia. During his PhD, he studied the effects of the appetite-suppressing hormone leptin on regulating glucose and lipid metabolism. Dr. Huynh completed his post-doctoral training at Duke University Medical Center, where he studied the metabolic functions of a family of enzymes called sirtuins, which are deacylase enzymes and thought to have anti-aging properties. At San José State University, Dr. Huynh continues to investigate how nutrients are metabolized and how this contributes to both normal and pathological processes.
Dr. Huynh is also passionate about teaching. He has extensive experience teaching physiology labs. Dr. Huynh is constantly striving to find better ways to teach physiology through methods that encourage critical thinking and development of practical skills. His current courses are listed below:
Biol 125 - Systems Physiology Lab
Biol 131 - Endocrine Physiology
Biol 178 - Integrative Physiology
LAB PHOTOS
CONTACT US
SHIPPING ADDRESS:
San Jose State University
Biology Department
ATTN: Dr. Frank Huynh
129 South 10th Street
San Jose, CA 95192-0100
The Huynh Lab is currently accepting applications for graduate students and undergraduate students.
If you are interested, contact Dr. Huynh via email with your CV, unofficial transcript, expected graduation date, long-term career goals, and why you want to join the Huynh Lab.